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Overall sequencing run performance is evaluated by determining whether the sequencing run meets the Illumina specifications for quality scores and data output. The actual run performance will vary based on sample type, quality, and clusters passing filter. Specifications are based on the Illumina PhiX control library at supported cluster densities. (Taken from https://support.illumina.com/bulletins/2019/10/does-my-sequencing-run-look-good-.html)

Salient Genomics is dedicated to providing high-quality service and offering flexible options that allow personalized service to our users. The genomics core offers Illumina Sequencing using the Illumina NextSeq 500 and Illumina MiSeq sequencing platforms. We are equipped to handle most sample types and workflows offering a variety of library preparation solutions, sequencing, and data analysis solutions for investigating the whole genome, exome, transcriptome, and targeted sequences.

To ensure a successful sequencing run, the sample must meet sufficient purity, quantity, and quality standards. Your submitted samples will be checked to meet these standards, and this QC assessment is included in the library prep price for each submitted sample that goes on to be prepared for sequencing. If the submitted sample needs to be improved, however, we are happy to work with the investigator to reach the necessary standards. Any additional QC assessment will be charged according to the currently posted pricing sheet.

We perform Illumina NGS library preparation from a variety of starting materials, including genomic DNA, PCR products, BACs, total RNA, mRNA. These NGS libraries could be sequenced with various Illumina’s platforms such as the MiSeq Personal System, NextSeq 500, NovaSeq6000, etc. Applications include de novo sequencing, resequencing of whole genomes and target DNA regions, sequencing of individual chloroplast genomes and metagenomics environmental samples, RNA analysis, and many more. (https://www.k-state.edu/igenomics/services/ngslibrary-prep.html)